Barrier Battut et al, 2016 identified in vitro parameters that are correlated with the fertility of stallion semen. These included among other things progressive motility and straight line, viable sperm, and morphology. Different studies compared INRA 96 efficiency with other stallion semen extenders. INRA 96 has consistently shown superior or at least equivalent performance to other extenders such as skimmed milk or homemade media.
Here is a list of different results you can read in those publications:
- Sperm progressive motility is significantly better in INRA 96 than in skimmed milk or homemade medium, and at least equivalent in casein based medium up to 48h to 72h of storage. (Batellier et al, 2001 & LeFrapper et al, 2010 & Fedorka et al, 2014 & Rečková et al, 2022)
- Sperm viability (or Sperm Intact Membrane) is significantly better in INRA 96 than in skimmed milk or homemade medium, and some casein based medium up to 48h of storage. (Rečková et al, 2022)
- Sperm cells morphology is better in INRA 96 and Casein-based medium than in skimmed milk-based medium (Rečková et al, 2022)
These results indicate that INRA 96 is an efficient solution for semen preservation in equine reproduction, as it helps maintain sperm quality and function during storage and transport, resulting in improved fertility outcomes compared to skimmed milk medium (Batellier et al, 2001).
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