How a flow cytometer works?


If you've had the chance to read the article Flow Cytometry Demystified: From Components to Applications in Semen Analysis, you're already well-acquainted with what a flow cytometer is and its constituent parts. In this second article, we will delve into the operational details of this technology and explain why it stands as an all-encompassing solution for your laboratory needs. 

How does it work?

Now that we understand the essential components of a flow cytometer, let's explore how it operates to analyze spermatozoa efficiently. Here are the main steps in the process:


1. Fluorescence Incubation: Before analysis, the sample is often treated with fluorescent dyes that bind to specific cellular components. These dyes emit fluorescence when exposed to laser light.


2. Laser Excitation: The sample is injected into the flow cytometer's capillary system. The laser, usually emitting light at 488 nm, is directed at the flowing particles. When the laser beam hits a fluorescently labeled particle, it excites the dye molecules, causing them to emit fluorescent light.


3. Detection Channels: Inside the flow cytometer, different receptors are used to capture the emitted light. These receptors are divided into channels, including the Forward Scatter Channel (FSC) for analyzing cell size and the Side Scatter Channel (SSC) for evaluating cell granularity. Additionally, various receptors are dedicated to analyzing the fluorescence emitted by the dyes, allowing for a detailed characterization of the semen sample.


By collecting data from these detectors, the flow cytometer generates a multi-dimensional profile of the spermatozoa in the sample, offering valuable information.


A Turnkey Solution for Your Lab

At IMV Technologies, we are committed to providing comprehensive solutions to meet your laboratory needs. When it comes to flow cytometry, we offer a turnkey solution that includes:


✔ A Range of Cytometers: We provide a diverse range of cytometers, including automated plate-based systems, single-tube analyzers, and configurations with different laser options. This ensures that you have the right instrument for your specific applications.

✔ Specific Software: Our dedicated software comes with templates tailored to semen analysis, making data analysis more efficient and accurate.


✔ Technical Support: Our team of experts is always on hand to offer technical support, installation and training, ensuring that your flow cytometer operates smoothly and that you can make the most of its capabilities.


✔ Ready-to-Use Kits: To simplify your research, we offer ready-to-use kits for various parameters, including viability, concentration, mitochondrial activity, oxidation, acrosome integrity, and bacterial concentration. These kits streamline your workflow and guarantee reliable results.

To learn more about flow cytometry and its benefits, join Our Webinar on September 26th

To gain a deeper understanding of how flow cytometers work and their applications in predicting fertility across different species, join us on the 26th of September. We will provide illustrations and in-depth explanations of this equipment. Moreover, we will demonstrate how flow cytometry can be employed effectively in semen analysis. Don't miss this opportunity to enhance your knowledge and expertise in this vital field.

We have prepared two sessions, you can join us no matter where you are 🌐:

💻 Session 1
Tuesday, 26th September
10h - 11h CEST 
Enroll here: 

 💻 Session 2
Tuesday, 26th September
17h - 18h CEST 
Enroll here:

Thank you for choosing IMV Technologies as your trusted partner in advancing your laboratory research and analysis capabilities. We look forward to seeing you at the webinar and assisting you in achieving your scientific goals.

Mélina Craus
Semen Analysis Product Manager
IMV Technologies