Flow Cytometry Demystified: From Components to Application in Semen Analysis


In the world of scientific inquiry and laboratory analysis, the flow cytometer stands as a beacon of innovation. These advanced instruments have reshaped the way we examine cells, offering unmatched precision and insight.

In this article, we will unravel the inner workings of the flow cytometer, starting with its fundamental components. But knowledge alone isn't our goal; we aim to provide you with a complete laboratory solution. From various cytometer options and specialized software to expert support and ready-to-use kits, we're here to empower your semen analysis and research!

What is a Flow Cytometer?

In the realm of modern laboratory technology, flow cytometers are indispensable tools that have revolutionized the way we analyze cells and particles. These sophisticated instruments are essential for a wide range of applications, from immunology and microbiology to clinical diagnostics but also for semen analysis. Let's delve into what exactly a flow cytometer is and its key components.


Components of a Flow Cytometer:

1. Laser: The heart of a flow cytometer is its laser, which serves as the light source for exciting the samples. Typically, a laser with a wavelength of 488 nm is used for this purpose.

2. Capillary: The capillary system is responsible for presenting the samples in front of the laser. It ensures that each particle or cell passes through the laser beam one at a time, allowing for precise analysis.

3. Filter: Filters in the flow cytometer play a crucial role in selecting and routing specific wavelengths of light emitted by the sample. These filters help isolate the fluorescence signals from the sample's background noise.

4. Detectors: Flow cytometers are equipped with detectors, which include photodiodes and photomultiplier tubes (PMT). These detectors capture the emitted light from the sample and convert it into electrical signals.

5. Electronics and Peripheral Computer System: The electronics and computer systems are at the core of data acquisition and analysis. They convert the signals from the detectors into digital data and perform the necessary analyses to generate meaningful results.

In essence, a flow cytometer is a highly advanced machine that combines precise components to provide comprehensive insights into the characteristics of cells and particles in a sample.

To learn more about flow cytometry and its benefits, join Our Webinar on September 26th

To gain a deeper understanding of how flow cytometers work and their applications in predicting fertility across different species, join us on the 26th of September. We will provide illustrations and in-depth explanations of this equipment. Moreover, we will demonstrate how flow cytometry can be employed effectively in semen analysis. Don't miss this opportunity to enhance your knowledge and expertise in this vital field.

We have prepared two sessions, you can join us no matter where you are 🌐:

💻 Session 1
Tuesday, 26th September
10h - 11h CEST 
Enroll here:
💻 Session 2
Tuesday, 26th September
17h - 18h CEST 
Enroll here:

Thank you for choosing IMV Technologies as your trusted partner in advancing your laboratory research and analysis capabilities. We look forward to seeing you at the webinar and assisting you in achieving your scientific goals.

Mélina Craus
Semen Analysis Product Manager
IMV Technologies